Name: Bacterial Gene Expression Technologies (en Inglés)
SKU: 48910568
Price: 51.21 USD
Availability: InStock
Author: Mahmoud Zaky; Merna Abou-Selima

portada Bacterial Gene Expression Technologies (en Inglés)

Bacterial Gene Expression Technologies (en Inglés)

Formato

Libro Físico

Editorial

Lap Lambert Academic Publishing

Autor

Mahmoud Zaky; Merna Abou-Selima

Año

2017

Idioma

Inglés

N° páginas

124

Encuadernación

Tapa Blanda

ISBN13

9786202004787

Bacterial Gene Expression Technologies (en Inglés)

Mahmoud Zaky; Merna Abou-Selima (Autor) · Lap Lambert Academic Publishing · Tapa Blanda

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Reseña del libro "Bacterial Gene Expression Technologies (en Inglés)"

Random integration of the IVET vector into the pathogen chromosome is performed by insertion–duplication mutagenesis to create a pool of recombinant pathogens (this means that the gene in which the vector has inserted by homologous recombination is not disrupted). Recombinants can be selected using antibiotic resistance, as an additional marker is also on the integrated IVET construct. Pooled clones are then inoculated into the mouse. The two main types of IVET promoter trap strategies are (a) the complementation of auxotrophic mutation and (b) the expression of antibiotic resistance. Only those bacteria that contain the selective marker fused to a gene that is transcriptionally active in the host are able to survive. After a suitable infection period, bacteria that express the marker are isolated from lung, liver, spleen, and mesenteric tissue.