Name: Base Excision Repair Pathway. Methods and Protocols
SKU: 64791408
Price: 132.87 USD
Availability: InStock
Author: Kishor K. Bhakat;Tapas K. Hazra

portada Base Excision Repair Pathway. Methods and Protocols

Base Excision Repair Pathway. Methods and Protocols

Formato

Libro Físico

Editorial

Humana

Autor

Kishor K. Bhakat;Tapas K. Hazra

Colección

Methods in Molecular Biology

Año

2024

N° páginas

262

Encuadernación

Tapa Blanda

Dimensiones

25.4x17.8 cm

ISBN13

9781071633755

Base Excision Repair Pathway. Methods and Protocols

Kishor K. Bhakat;Tapas K. Hazra (Autor) · Humana · Tapa Blanda

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Reseña del libro "Base Excision Repair Pathway. Methods and Protocols"

This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.