Scientific knowledge, in all its aspects, is widely understood to be shaped by social and material infrastructures, as well as by the practices unfolding within these arrangements. Considering ongoing, particularly AI-driven infrastructural transformation and digitization of epistemic cultures, the question of how infrastructures and practices shape what counts as (non-)knowledge arises anew. The interdisciplinary contributions to this volume offer a broad and nuanced insight into the interrelations between knowledge practices and their infrastructures, including libraries, Wikipedia, human bodies, digital corpora, and universities. Together, they foreground the complexity of knowledge, and invite readers to critically reconsider prevailing epistemic regimes.