Mycobacterium Ulcerans: Methods and Protocols (Methods in Molecular Biology) (en Inglés)
Reseña del libro "Mycobacterium Ulcerans: Methods and Protocols (Methods in Molecular Biology) (en Inglés)"
Preface...Table of Contents...Contributing Authors...Part I Mycobacterium ulcerans Disease and Host Pathogen Interaction1. Overview: Mycobacterium ulcerans Disease (Buruli ulcer)Katharina Röltgen and Gerd Pluschke2. Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin TissueMarie-Thérèse Ruf3. Primary Isolation of Mycobacterium ulceransDorothy Yeboah-Manu, Adwoa Asante-Poku, and Samuel Yaw Aboagye4. Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon ResonanceChristopher J. Day, Jessica Poole, Gerd Pluschke, and Michael P. Jennings5. Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans CultureMélanie Foulon, Estelle Marion, and Laurent Marsollier6. DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCRCaroline J. Lavender and Janet A.M. Fyfe7. Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulceransJanet A.M. Fyfe and Caroline J. Lavender8. Detection of Mycobacterium ulcerans DNA using Real-Time PCR Janet A.M. Fyfe and Caroline J. Lavender9. Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCRMarcus Beissner, Richard Odame Phillips, and Gisela Bretzel10. Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future DirectionsJoseph W. S. Timothy, Rachel L. Pullan, and Rie R. Yotsu Part II Purification and Quantification of Myolactone 11. Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulceransKatharina Röltgen and Gerd Pluschke12. Conditions for Handling and Optimal Storage of MycolactoneJessica Z. Kubicek-Sutherland, Basil I. Swanson, and Harshini Mukundan13. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples Aline Rifflet, Caroline Demangel, and Laure Guenin-Macé14. Detection of Mycolactone by Thin Layer ChromatographyRichard Kwamla Amewu and Thomas Spangenberg15. Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans MycolactoneLouisa Warryn16. Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61Sarah O'Keefe, Stephen High, and Caroline DemangelPart III Drug Development against Mycobacterium ulcerans17. Overview: Development of Drugs against Mycobacterium ulceransGerd Pluschke and Katharina Röltgen18. In Vitro Assessment of Drug Activity against Mycobacterium ulceransOliver D. Komm, Deepak V. Almeida, Paul J. Converse, and Eric L. Nuermberger19. Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcerPaul J. Converse, Deepak V. Almeida, and Eric L. Nuermberger20. Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulceransSangeeta Susan Thomas, Nitin Pal Kalia, and Kevin Pethe21. Determination of Bioenergetic Parameters in Mycobacterium ulceransSangeeta Susan Thomas and Kevin PetheSubject Index List...
