Pirna: Methods and Protocols (Methods in Molecular Biology, 2509) (en Inglés)
Reseña del libro "Pirna: Methods and Protocols (Methods in Molecular Biology, 2509) (en Inglés)"
Part I: Methods to Study the Nature and Function of piRNAs in Non-Classical Organisms 1. Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides Rebecca Halbach and Pascal Miesen 2. CRISPR-Mediated Genome Engineering in Aedes aegypti Ruichen Sun, Ming Li, Conor J. McMeniman, and Omar S. Akbari 3. PIWI-Directed DNA Elimination for Tetrahymena Genetics Salman Shehzada and Kazufumi Mochizuki 4. Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing Makoto Kashima, Atsumi Miyata, and Norito Shibata 5. Isolation and Processing of Bovine Oocytes for Small RNA Sequencing Minjie Tan, Helena T.A. van Tol, and Elke F. Roovers 6. 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther Koji Koizumi and Reina Komiya Part II: Methods to Study Roles of piRNAs in Classic Model Organisms 7. Cloning, Sequencing, and Linkage Analysis of piRNAs Rippei Hayashi 8. Drosophila Genetic Resources for Elucidating piRNA Pathway Kuniaki Saito 9. Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System Chikara Takeuchi, Kensaku Murano, Mitsuru Ishikawa, Hideyuki Okano, and Yuka W. Iwasaki Part III: Methods to Study Nuclear Regulation by Other Non-Coding RNAs 10. Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline Valeriya Morgunova, Maria M. Sukhova, and Alla Kalmykova 11. CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells Akihiko Sakashita, Masaru Ariura, and Satoshi H. Namekawa 12. Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability Mariko Dacher, Risa Fujita, Tomoya Kujirai, and Hitoshi Kurumizaka 13. Revisiting the Glass Treatment for Single Molecule Analysis of ncRNA Function Shuting Shen, Masahiro Naganuma, Yukihide Tomari, and Hisashi Tadakuma 14. Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification Wan Kin Au Yeung and Hiroyuki Sasaki 15. Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq Abd-El Monsif Shawky, Mahmoud Dondeti, Zissimos Mourelatos, and Anastasios Vourekas 16. UPA-Seq-Based Search Method for Functional lncRNA Ca
