Poly(Adp-Ribose) Polymerase: Methods and Protocols (Methods in Molecular Biology, 2609) (en Inglés)
Reseña del libro "Poly(Adp-Ribose) Polymerase: Methods and Protocols (Methods in Molecular Biology, 2609) (en Inglés)"
Part I: Detection and Quantification of pADPr 1. Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K.L. Leung 2. Detecting and Quantifying pADPr In Vivo Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hülya Bayır, and Robert S.B. Clark 3. Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol 4. Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose) Johannes Rudolph and Karolin Luger 5. Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus 6. An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa 7. Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy Lukas Muskalla, Anka Güldenpfennig, and Michael O. Hottiger 8. A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery Johannes Gregor Matthias Rack and Ivan Ahel Part II: Identification of Protein Targets 9. Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies Helen Dauben and Ivan Matic 10. A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins Dongsheng Yao, Heba Ahmed, and Junqi Song 11. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus Part III: Functional Analysis 12. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer Nura Lutfi, Carlos Martínez, and José Yélamos 13. Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death Hannah K. Hall and David W. Koh 14. Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function Tünde Kovács, Boglárka Rauch, Edit Mikó, and Péter Bai 15. Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrome
