Name: RP-HPLC Method Development and Validation (en Inglés)
Price: 73.53 USD
Availability: InStock
Author: Hechhu, Ramana ; Balla, Tamrat Balcha ; Vasanthi, R.
ISBN: 9786203306927

portada RP-HPLC Method Development and Validation (en Inglés)

RP-HPLC Method Development and Validation (en Inglés)

Formato

Libro Físico

Autor

Ramana Hechhu

Tamrat Balcha Balla

R. Vasanthi

Editorial

LAP Lambert Academic Publishing

Idioma

Inglés

N° páginas

112

Encuadernación

Tapa Blanda

Dimensiones

22.9 x 15.2 x 0.7 cm

Peso

0.18 kg.

ISBN13

9786203306927

Categorías

Farmacología

RP-HPLC Method Development and Validation (en Inglés)

Ramana Hechhu (Autor) · Tamrat Balcha Balla (Autor) · R. Vasanthi (Autor) · LAP Lambert Academic Publishing · Tapa Blanda

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Reseña del libro "RP-HPLC Method Development and Validation (en Inglés)"

High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Thiocolchicoside and Lornoxicam was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer mixed in the ratio of 70:30 % v/ v. Inertsil C18 column C18 (4.6 x 150mm, 5μm) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 260 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the linearity range of Thiocolchicoside and Lornoxicam were found to be from 100-500 μg/ml of Thiocolchicoside and 1-5μg/ml of Lornoxicam . Linear regression coefficient was not more than 0.999.The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of Thiocolchicoside and Lornoxicam . LOD and LOQ were found to be within limit.The results obtained on the validation parameters met ICH and USP requirements .it inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application.