Name: Effect of miR-34a replacement (en Inglés)
SKU: 57222301
Price: 68.38 USD
Availability: InStock
Author: Jafarpour, Nazanin ; Sina, Mohammad
ISBN: 9786138918455

portada Effect of miR-34a replacement (en Inglés)

Effect of miR-34a replacement (en Inglés)

Formato

Libro Físico

Autor

Nazanin Jafarpour

Mohammad Sina

Editorial

Scholars' Press

Idioma

Inglés

N° páginas

Encuadernación

Tapa Blanda

Dimensiones

22.9x15.2x0.5 cm

Peso

0.14 kg.

ISBN13

9786138918455

Categorías

Medicina: cuestiones generales

Effect of miR-34a replacement (en Inglés)

Nazanin Jafarpour (Autor) · Mohammad Sina (Autor) · Scholars' Press · Tapa Blanda

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Reseña del libro "Effect of miR-34a replacement (en Inglés)"

Colorectal cancer as the most common cancer of the digestive tract is the fourth leading cause of death in the world. According to studies, miRNA-34a (miR-34a) expression declines in colon cancer. The aim of this study was to investigate the effect of miR-34a on growth and migration inhibition and apoptosis induction of colon cancer cells. In this experimental study, HCT116 human colorectal cancer cells were used in RPMI1640 medium. MiR-34a transmission into cancer cells was performed by the jetPEI transfection reagent. MTT assay to determine the survival of cancer cells and the qRT-PCR test to confirm the transfer of miR-34a to the cells and also an investigation of the expression of Caspase genes were used. Finally, the Wound healing test in order to investigate the migration status of transfected and control cells was performed. The expression changes of the intended genes in the transfected cells were compared with the control cells using the 2-ΔΔCt method.